Technology Research & Development

Drs Mark Le Gros and former NCXT Postdoc Bertrand Cinquin standing outside the soft x-ray microscope room at the ALS. Photo by Sheraz Sadiq / QUEST

Using soft x-ray microscopy we can image an entire cell in its natural hydration state. Determining the location of specific protein-protein interactions requires proteins are labeled with readily detectable markers, either in the soft x-ray microscope, or in another modality and the data correlated. The latter is the preferred option since direct visualization of a protein using soft x-rays requires it be labeled with a heavy metal tag (a potentially damaging process to the integrity of the cell structure and molecular organization). Consequently, we have focused our technology development towards correlated microcopy, in particular correlated cryogenic confocal microscopy. This technique is described in the below manuscript, followed by a recent research application. You can download pdf version of these papers here and here)

A correlated imaging study of the Inactive X chromosome, in collaboration with the Panning Group (an NCXT driving biomedical project).