Soft X-Ray Tomography Reveals Gradual Chromatin Compaction and Reorganization during Neurogenesis In Vivo

Posted by admin
TitleSoft X-Ray Tomography Reveals Gradual Chromatin Compaction and Reorganization during Neurogenesis In Vivo
Publication TypeJournal Article
Year of Publication2016
AuthorsLe Gros MA, Clowney EJ, Magklara A, Yen A, Markenscoff-Papadimitriou E, Colquitt B, Myllys M, Kellis M, Lomvardas S, Larabell CA
Journal TitleCell Rep
Volume17
Pages2125-2136
Journal DateNov 15
ISBN Number2211-1247 (Electronic)
Accession Number27851973
Keywordschromatin, differentiation, neurogenesis, nuclear organization, nucleus, olfactory sensory neurons, soft x-ray tomography
Abstract

The realization that nuclear distribution of DNA, RNA, and proteins differs between cell types and developmental stages suggests that nuclear organization serves regulatory functions. Understanding the logic of nuclear architecture and how it contributes to differentiation and cell fate commitment remains challenging. Here, we use soft X-ray tomography (SXT) to image chromatin organization, distribution, and biophysical properties during neurogenesis in vivo. Our analyses reveal that chromatin with similar biophysical properties forms an elaborate connected network throughout the entire nucleus. Although this interconnectivity is present in every developmental stage, differentiation proceeds with concomitant increase in chromatin compaction and re-distribution of condensed chromatin toward the nuclear core. HP1beta, but not nucleosome spacing or phasing, regulates chromatin rearrangements because it governs both the compaction of chromatin and its interactions with the nuclear envelope. Our experiments introduce SXT as a powerful imaging technology for nuclear architecture.

URLhttps://www.ncbi.nlm.nih.gov/pubmed/27851973http://ac.els-cdn.com/S2211124716314802/1-s2.0-S2211124716314802-main.pdf?_tid=2d1d3fb0-f47f-11e6-a976-00000aab0f26&acdnat=1487273953_5708a918261d03084b596cc27367b816