Mesoscale imaging with cryo-light and X-rays: Larger than molecular machines, smaller than a cell

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TitleMesoscale imaging with cryo-light and X-rays: Larger than molecular machines, smaller than a cell
Publication TypeJournal Article
Year of Publication2017
AuthorsEkman AA, Chen JH, Guo J, McDermott G, Le Gros MA, Larabell CA
Journal TitleBiol Cell
Volume109
Pages24-38
Journal DateJan
ISBN Number1768-322X (Electronic)0248-4900 (Linking)
Accession Number27690365
KeywordsAnimals, Correlated imaging, Cryogenic, Cryopreservation/methods, fluorescence, Humans, Imaging, Three-Dimensional/methods, Microscopy, Fluorescence/methods, Microscopy/*methods, Molecular Imaging/methods, soft x-ray, tomography, Tomography, X-Ray/*methods
Abstract

In the context of cell biology, the term mesoscale describes length scales ranging from that of an individual cell, down to the size of the molecular machines. In this spatial regime, small building blocks self-organise to form large, functional structures. A comprehensive set of rules governing mesoscale self-organisation has not been established, making the prediction of many cell behaviours difficult, if not impossible. Our knowledge of mesoscale biology comes from experimental data, in particular, imaging. Here, we explore the application of soft X-ray tomography (SXT) to imaging the mesoscale, and describe the structural insights this technology can generate. We also discuss how SXT imaging is complemented by the addition of correlative fluorescence data measured from the same cell. This combination of two discrete imaging modalities produces a 3D view of the cell that blends high-resolution structural information with precise molecular localisation data.

URLhttps://www.ncbi.nlm.nih.gov/pubmed/27690365http://onlinelibrary.wiley.com/store/10.1111/boc.201600044/asset/boc201600044.pdf?v=1&t=iz8sef6u&s=f853be5b72b28599c5ed22301ff922e072af0052