Soft X-ray microscopy to 25 nm with applications to biology and magnetic materials

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TitleSoft X-ray microscopy to 25 nm with applications to biology and magnetic materials
Publication TypeJournal Article
Year of Publication2001
AuthorsDenbeaux G, Anderson E, Chao W, Eimuller T, Johnson L, Kohler M, Larabell C, Legros M, Fischer P, Pearson A, Schultz G, Yager D, Attwood D
Journal TitleNuclear Instruments & Methods in Physics Research Section a-Accelerators Spectrometers Detectors and Associated Equipment
Volume467
Pages841-844
Journal DateJul 21
ISBN Number0168-9002
Accession NumberISI:000171012800002
Keywordscryogenic preservation, high resolution, magnetic imaging, X-ray microscopy
Abstract

We report both technical advances in soft X-ray microscopy (XRM) and applications furthered by these advances. With new zone plate lenses we record test pattern features with good modulation to 25 nm and smaller. In combination with fast cryofixation, sub-cellular images show very fine detail previously seen only in electron microscopy, but seen here in thick, hydrated, and unstained samples. The magnetic domain structure is studied at high spatial resolution with X-ray magnetic circular dichroism (X-MCD) as a huge element-specific magnetic contrast mechanism. occurring e.g. at the L-2.3 edges of transition metals. It can be used to distinguish between in-plane and out-of-plane contributions by tilting the sample. As XRM is a photon based technique, the magnetic images can be obtained in unlimited varying external magnetic fields. The images discussed have been obtained at the XM-1 soft X-ray microscope on beamline 6.1 at the Advanced Light Source in Berkeley. (C) 2001 Elsevier Science B.V. All rights reserved.

URL<Go to ISI>://000171012800002