Nuclear aggregation of olfactory receptor genes governs their monogenic expression

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TitleNuclear aggregation of olfactory receptor genes governs their monogenic expression
Publication TypeJournal Article
Year of Publication2012
AuthorsClowney EJ, Le Gros MA, Mosley CP, Clowney FG, Markenskoff-Papadimitriou EC, Myllys M, Barnea G, Larabell CA, Lomvardas S
Journal TitleCell
Volume151
Pages724-37
Journal DateNov 9
ISBN Number1097-4172 (Electronic)0092-8674 (Linking)
Accession Number23141535
Abstract

Gene positioning and regulation of nuclear architecture are thought to influence gene expression. Here, we show that, in mouse olfactory neurons, silent olfactory receptor (OR) genes from different chromosomes converge in a small number of heterochromatic foci. These foci are OR exclusive and form in a cell-type-specific and differentiation-dependent manner. The aggregation of OR genes is developmentally synchronous with the downregulation of lamin b receptor (LBR) and can be reversed by ectopic expression of LBR in mature olfactory neurons. LBR-induced reorganization of nuclear architecture and disruption of OR aggregates perturbs the singularity of OR transcription and disrupts the targeting specificity of the olfactory neurons. Our observations propose spatial sequestering of heterochromatinized OR family members as a basis of monogenic and monoallelic gene expression. PAPERCLIP:

URLhttp://www.ncbi.nlm.nih.gov/pubmed/23141535http://ac.els-cdn.com/S009286741201286X/1-s2.0-S009286741201286X-main.pdf?_tid=2d4269b4-61af-11e2-855c-00000aab0f27&acdnat=1358541758_8deb83b5b2c52fa2e88a64bc88a019a5http://ac.els-cdn.com/S009286741201286X/1-s2.0
Alternate JournalCell