Visualizing and quantifying cell phenotype using soft X-ray tomography

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TitleVisualizing and quantifying cell phenotype using soft X-ray tomography
Publication TypeJournal Article
Year of Publication2012
AuthorsMcDermott G, Fox DM, Epperly L, Wetzler M, Barron AE, Le Gros MA, Larabell CA
Journal TitleBioessays
Volume34
Pages320-327
Journal DateApr
ISBN Number0265-9247
Accession NumberWOS:000301434700016
Keywords3d reconstruction, biological applications, cellular, correlated, cryo-light, Cryoelectron tomography, Electron tomography, fluorescent-probes, Microscopy, molecules, morphology, multi-modal, nanoscale, resolution, three-dimensional
Abstract

Soft X-ray tomography (SXT) is an imaging technique capable of characterizing and quantifying the structural phenotype of cells. In particular, SXT is used to visualize the internal architecture of fully hydrated, intact eukaryotic and prokaryotic cells at high spatial resolution (50?nm or better). Image contrast in SXT is derived from the biochemical composition of the cell, and obtained without the need to use potentially damaging contrast-enhancing agents, such as heavy metals. The cells are simply cryopreserved prior to imaging, and are therefore imaged in a near-native state. As a complement to structural imaging by SXT, the same specimen can now be imaged by correlated cryo-light microscopy. By combining data from these two modalities specific molecules can be localized directly within the framework of a high-resolution, three-dimensional reconstruction of the cell. This combination of data types allows sophisticated analyses to be carried out on the impact of environmental and/or genetic factors on cell phenotypes.

URL<Go to ISI>://WOS:000301434700016
Alternate JournalBioessaysBioessays