CryoLight Microscopy

Imaging the architecture of a cell typically only provides partial answers to questions being posed. A full understanding also usually requires information on the locations of key molecules inside the cell. It is possible to label molecules with heavy metals, and view them directly in situ in a soft x-ray tomogram. However, this process involves the use of protocols that frequently compromise the integrity of the specimen. Therefore, we have developed high numerical aperture cryogenic light microscopy as a means of carrying out correlated imaging; x-ray imaging to visualize the sub-cellular structure, and fluorescence imaging for localizing tagged molecules within this framework. The prototype cryolight microscope is described below (download pdf here).